CBlock Clinical Studies

January 11, 2000 

Structure and an alpha-amylase inhibitor produced by marine actinomycete and its lowering effects in vivo of glucose and lipid in blood.

Suetsuna, K.; Nagatomo, K.; Doi, K.

Journal of Shimonoseki University of Fisheries, vol. 42
January 11, 2000

A fraction of alpha-amylase inhibitor was extracted from the mycelia of a marine Actinomycetes, Streptomyces sp. No.2020 found in seawater. The fraction was purified using activated charcoal and Dowex 50W column chromatography. The basic structure of the fraction of alpha-amylase inhibitor (AI-2020) was identified as oxireno (e) pyrrolo (2, 1-b) benzoxazole-2, ,3, 5, 6-tetraol, decahydro-7- (1-hydroxyethyl) 1a- (hydroxymethyl) -, structure forumla 11, on the basis of the elementary analysis, mass spectrum, infrared (IR) and nuclear magnetic resonance (NMR) spectra of the hydrolyzate of AI-2020. The AI-2020 suppressed the elevation of blood glucose in vivo after oral administration of starch. In streptozotocin diabetic rats receiving AI-2020, triglyceride, free fatty acid, total cholesterol and syndrome including symptoms such as hyperglycemia. These findings indicate that AI-2020 may be useful for control of some carbohydrate dependent diseases such as diabetes, obesity and hyperlipemia.